Mechanisms of miR-326-5p/LCN2 signaling axis in cerebral ischemia pre-adaptation and post-adaptation to improve neuronal apoptosis

Abstract

Post-ischemic adaptation and pre-adaptation have been recognized as effective neuroprotective tools, but the mechanism is not clear. In this paper, through the preliminary experiments, it was found that autonomous running wheel pre-adaptation could promote post-ischemic adaptation and effectively inhibit neuronal apoptosis induced by cerebral ischemia/reperfusion in mice. After high-throughput screening and luciferase experiments, the article confirmed that miR-326-5p/LCN2 signaling axis mediated the protection of neuronal damage under ischemia-reperfusion conditions by the above two means. In this project, this article intends to investigate in detail the specific molecular mechanisms and signaling pathways of miR-326-5p/LCN2 signaling axis for neuroprotection under post-adaptive and pre-adaptive binding conditions by immunoprecipitation, immunohistochemistry, database screening, apoptosis assay, and other technological means. Combined with in vivo animal experiments and isolated cell experiments, we verified the mechanism of miR-326-5p/LCN2 signaling axis and its downstream effector molecules in the process of post-adaptation and pre-adaptation to ameliorate neuronal apoptosis. This study is expected to provide new theoretical support for the protective mechanism of pre-adaptation and post-adaptation on brain tissue during ischemia. At the same time, it will provide a new theoretical basis for clinical understanding of the pathogenesis of cerebral ischemia and the development of diagnostic and therapeutic strategies.