Relationship between β3 adrenergic receptor gene polymorphism and non-alcoholic fatty liver disease and its risk factors

Abstract

Objective: Detection of Beta-3 adrenergic receptor (β-3-AR) gene polymorphism distribution in patients with nonalcoholic fatty liver disease and healthy people, and exploration the polymorphisms of the β-3-AR gene and non-alcoholic fatty liver。

Methods: From October 2021to August 2022, in health medical center in first people's Hospital of Yunnan province Kunming city, we examined 336 people of Han population. There are 181 healthy people taken as control group, and 155 people with fatty liver as case goup who were confermed by abdominal b-ultrasound detection, without alcohol, drugs, viruses, and other compelling reason can result in liver damage. The physical index in the two groups were recorded, including height, weight, waist and hip circumference, blood pressure, heart rate, biochemical parameters (including liver function, kidney function, fasting blood glucose, blood lipids), and abdominal b-Ultrasound results (including liver, gall bladder, pancreas, spleen and two kidneys) . Polymerase chain reaction (PCR) and sequencing were used to detect the polymorphisms in Beta 3 adrenaline receptor gene. The differences of the gene polymorphisms between the two groups were analyzed as well as some clinic parameters. The SPSS17.0 package was used for data analyzing. The alleles frequencies in both groups were agree with Hardy-Weiberg equilibrium. The genotype and allele frequency in each group were analyzed by the χ² test (P>0.05). Primary outcome measures were carried out normality test, after logarithmic transformation for a skewed distribution of information similar to the normal distribution; quantitative data were compared using t test, qualitative data using χ²; with P<0.05 as significant.

Results

1.336 individuals, among which 181 were healthy controls, and 155 were non-alcoholic fatty liver cases, overall prevalence of 46.1%; male prevalence 114 cases, the prevalence rate is 51.1%; women in 41 cases, prevalence rate of 36.5%. Prevalence of males was significantly higher than women (χ ² = 6.64,P=0.01).

2.Non-alcoholic fatty liver patients BMI, total cholesterol, triglyceride and low-density lipoprotein, significantly higher than the control group, while HDL was significantly reduced (p <0.01).

3.Non-alcoholic fatty liver Trp64Trp, Trp64Arg and Arg64Arg frequencies are 69.6%, 28.7% and 1.7%; Arg and Trp allele frequencies are 84% and 16%. Normal healthy control Trp64Trp, Trp64Arg and Arg64Arg frequencies are 61.3%, 35.5% and 3.2%; Arg and Trp allele frequencies are 79% and 21%. Non-alcoholic fatty liver with a healthy control group comparison of genotypes and allele frequency, there were no significant differences (p >0.05).

4.Health control group within different gene type clinical information compared found (due to Arg/Arg gene type cases number less, so into Trp/Arg gene type for statistics), weight index, and gender, and age, and total cholesterol, and glycerol three fat, and low density lipoprotein cholesterol, and high-density lipoprotein cholesterol, clinical index in Trp/Trp gene type and Trp/Arg+ Arg/Arg gene type are no significantly sex differences (p >0.05).

5.Non-alcohol sex fat liver group within different gene type  clinical information compared found (due to Arg/Arg gene type cases number less, so into Trp/Arg gene type for statistics), weight index, and gender, and age, and total cholesterol, and glycerol three fat, and low density lipoprotein cholesterol, and high-density lipoprotein cholesterol, clinical index in Trp/Trp gene type and Trp/Arg+ Arg/Arg gene type are no significantly sex differences (p >0.05).

Conclusion

    1 .Non-alcoholic fatty liver disease prevalence wassignificantly higher than that of male female.

2 .Non-alcoholic fatty liver patients BMI index, total cholesterol, and triglycerides and low-density lipoprotein, significantly higher than the control group, and high density lipoprotein was reduced significantly.

3 .Non-alcoholic fatty liver with a healthy control group comparison of genotypes and allele frequency, there are no significant differences.

4 .Between different genotypes in the healthy control group body mass index, gender, age, total cholesterol, triglycerides, LDL cholesterol, HDL cholesterol and other clinical parameters in Trp/Trp gene Trp/Arg+ and Arg/Arg showed no significant difference between genotypes.

5 .Within the Group of non-alcoholic fatty liver clinical information clinical indicators among different genotypes in Trp/Trp Trp/Arg+ Arg/Arg genotype and genotype showed no significant difference between.