miR-124-3p alleviated the metastasis of Colorectal carcinoma through FOXQ1/ERK pathway
Keywords:
FOXQ1, Colorectal carcinoma, miR-124-3p, microRNAAbstract
Colorectal carcinoma (CRC) is currently one of the most common malignancies, accounting for approximately 10% of all new cases diagnosed worldwide each year. FOXQ1 regulates metabolic homeostasis, promotes epithelial cell differentiation, inhibits smooth muscle differentiation, mediates skin follicle development, and exerts immune functions against related diseases. However, few research has focus the relationship between miRNAs and FOXQ1 in CRC. In this manuscript, we investigated the mechanism of miR-124-3p on cell proliferation, apoptosis inhabitation, migration, and invasion by targeting FOXQ1/ERK pathway. SW480 was used for research, and hsa-miR-124-3p mimics and pcDNA-FOXQ1 and their negative control were transfected into SW480 cells. Cell viability was detected by CCK-8 and cell colony assay, apoptosis rate and cell cycle were detected by flow cytometry, migration index and invasion number were detected by wound healing assay and Transwell assay. The expression of FOXQ1 in tumor tissues and corresponding para tumor tissues was detected by qPCR and IHC. The expression of FOXQ1 and the activation of ERK/1/2 in SW480 were detected by western blot. The results showed that FOXQ1 was highly expressed in colorectal carcinoma tissues. AS a directly target of hsa-miR-124-3p, hsa-miR-124-3p hampered the cell proliferation, apoptosis inhibition, cell cycle process, cell migration and invasion, and tumor growth through FOXQ1. Also, hsa-miR-124-3p overexpression regulated ERK pathway by targeting FOXQ1. In conclusion, we found that miR-124-3p regulated cell proliferation, apoptosis inhibition, migration, invasion and tumor growth by targeting FOXQ1/ERK pathway. These results may indicate that FOXQ1 can be a potential target of early diagnosis and treatment of colorectal carcinoma.
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