Aminophylline inhibits the expression of inflammatory factors in airway smooth muscle cells under hypoxia through miR-138-5p/HIF1A axis

Abstract

Background:To study how aminophylline regulates miR-138-5p to target the expression of HIF1A to inhibit the expression of inflammatory factors in airway smooth muscle cells;

Methods:Use aminophylline and normal saline to treat human airway smooth muscle cells, respectively, and then use enzyme-linked immunoassay Adsorption test (ELISA) method to detect tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6) levels, real-time fluorescent quantitative PCR (RT-qPCR) to detect miR-138-5p and HIF1A expression levels, Use bioinformatics methods to screen the regulatory relationship of miR-138-5p/HIF1A, and verify the regulatory connection with a dual fluorescence report experiment. Use miR-138-5p inhibitors to act on airway smooth muscle cells to observe its effect on aminophylline—the impact of the role.

Results:Compared with the control group, the expression of miR-138-5p in the aminophylline group was significantly increased (p<0.05), the expression of HIF1A was significantly decreased (P<0.05), and the manifestation of TNF-α and IL-6 was significantly higher in the aminophylline group The control group decreased (P<0.01), bioinformatics screening suggested that miR-138-5p may have a regulatory relationship with HIF1A. The dual-fluorescence report showed that miR-138-5p might have a regulatory relationship with HIF1A.

Conclusions:Aminophylline can effectively inhibit the expression of inflammatory factors in airway smooth muscle cells under hypoxia. The mechanism may be through up-regulation of miR-138-5p and then down-regulation of HIF1A expression.