Therapeutic effect of Polygala crotalarioides on a middle cerebral artery occlusion/reperfusion (MCAO) modle
Keywords:
P. crotalarioides, Ischemic stroke, neuron apoptosisAbstract
Background:
Ischemic stroke is a devastating disease with high mortality and disability, causing a significant economic and social burden, and Middle Cerebral Artery Occlusion/reperfusion (MCAO/R) caused by ischemic stroke treatment causes severe brain injury. Polygala crotalarioides has shown medical potential as a highly-effective, hormone-free, natural anti-fatigue and cardiotonic agent, among the Wa ethnicity. Previous studies have shown that Polygala crotalarioides (P. crotalarioides) contains many active ingredients, but the pharmacological mechanism of it in MCAO/R is not investigated.
Methods:
The in vivo MCAO/R rat model was constructed. Modified Neurological Severity Scores (mNSS) and 2,3,5-triphenyltetrazolium chloride staining (TTC) were performed to reveal the protective role of P. crotalarioides after MCAO/R. The apoptotic proteins for western-blot assay were isolated from the hippocampus region of the brain. In order to reveal the proliferation of neurons and Nissl body, immunofluorescence (IF) assays and Nissl staining were performed in vitro, respectively.
Results:
Our results showed that P. crotalarioides treatment significantly suppressed neuronal apoptosis. Western-blot analysis indicated that the apoptosis-related Bax/Bcl-2 and cleaved-caspase-3 were decreased after P. crotalarioides treatment. The score of mNSS and the infract area in P. crotalarioides group were alleviated compared with the MCAO/R group. The results of Nissl staining suggested that Nissl body was enhanced under P. crotalarioides treatment. And the proliferation of neurons was positively regulated by P. crotalarioides.
Conclusions:
The present study suggested that P. crotalarioides exhibited neuroprotective effect that in MCAO/R, possibly through down-regulating the neuron apoptosis.
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